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KMID : 0357119920140010015
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Korean Journal of Immunology
1992 Volume.14 No. 1 p.15 ~ p.24
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Regulation of Cellular Immunity in Cryptococcosis Ⅲ. Induction of Cryptococcus neoformans-specific T Cells and Hybridoma
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하대유
박영민/박윤규/이한구/박종욱
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Abstract
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Cryptococcus neoformans is a major pathogen of lifethreatening opportunistic infections in normal and patients with impaired cell-mediated immunologic defense, particularly in those with acquired immunodeficiency syndorme. The cryptococcal(CneF)
antigen
induced a population of lymph node cells that. Upon adoptive transfer, could specifically suppress the afferent limb of delayed-type hypersensitivity(DTH) response to CneF antigen. Infection of C. neoformans-infected mouse serum also suppressed
the
DTH
reaction to CneF antigen. The suppression of DTH reaction to CneF antigen caused by intravenous injection of CneF antigen of C. neoformans infected mouse serum was somewhat recoved by pretreatment of 100mg/kg of cyclosporin A or cyclophosphamide.
Lymph
node cells(Ts1 cells) from CneF-injected mice can induce in the spleens of recipient mice a second-order suppressor cell population that suppresses the efferent limb of the DTH response. In addition, a soluble factor (TsF1) was extracted from the
Ts1
cells and the TsF1 could induce Ts2 cells. C. neopformans-specific Ts1 cell was succesfully hybridied and the culture supernatant(HTsP1) from the Ts1 hybridoma could also induce Ts2 cells. Taken together, these results suggest that complex
suppressor
cell circuit is present in cryptococcosis, and that cyclosporin A and cyclophosphamide can be utilized for acquisition of protective immunity in cryptococcosis.
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KEYWORD
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